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Matrix repair is impaired in the avascular IVD, resulting in weakening and increased vulnerability to damage by physiologic loading

A study has found that TMAO may induce pyroptosis in HUVECs by inhibiting ALDH2 activity, which subsequently activates the ROS/NLRP3/GSDMD pathway

The resultant 0.6 ml BHM solution containing cells was sonicated and centrifuged at 1,000 g for 10 min, and the supernatant was used for measuring the enzyme activity