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doi: 10.1111/j.1365-3040.2012.02564.x 32 GladyshevV

[9] If you do feel flush or itchy, it should be mild and short-lived

0.5 ml 0.1%, trichloroacetic acid (TCA), leaf extract supernatant, 0.5 ml of 100 mM potassium phosphate buffer, and 2 ml reagent (1 ml KI w/v double-distilled water) were added in the reaction mixture