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To study gene expression, total RNA was extracted from half-colon samples using phenol-chloroform methods and purified with lithium chloride precipitation, which is particularly important to remove residual DSS polysaccharides that can interfere with downstream reactions

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The existing findings from epigenetics research need to be appraised in the context of well-known technical limitations epigenetics, such as the blood-brain inconsistencies, tissue-type specificity (Bakulski, Halladay, Hu, Mill, & Fallin, Reference Bakulski, Halladay, Hu, Mill and Fallin2016

Thus, the brain is more susceptible to diseases like Alzheimers disease (AD), which is closely associated with oxidative damage