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The 3D structure of the GPx3 mutant with all Cys converted to Ser was refined by MD simulations and analyzed with Profile-3D and Procheck following the method as described previously 31,32

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Chromatographic separation was carried out using an UltiMate 3000 HPLC system (Thermo Fisher Scientific, Rockford, IL, USA) fitted with a YMC-Triart PFP column (3 mm particle size, 12 nm pore size, 150 2.1 mm