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Although in vitro models have provided valuable insights into the active metabolic networks of cancer cells, the metabolism of cells in culture is often different from the metabolism of tumors in vivo 21 , and metabolite profiling albeit informative, does not directly probe metabolic flux and thus incompletely characterizes metabolic pathway activity

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In IDH1 -mutant glioma, activation of AKT activated NRF2, reduced the cellular ROS and inhibited ferroptosis, thus promoted tumor progression [37]