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The soluble and insoluble fractions were separated by centrifugation for 45 min at 48,000 g

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Hepatocyte premature senescence is initiated by oxidative stress, along with reduction of IGF-1 during CCl 4 -induced liver fibrogenesis The area density of Masson staining and SA--gal staining showed that with time, CCl 4 initiated hepatic steatosis on Day 6 and the early stage of fibrosis on Day 28