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Meanwhile, a culture of EcN pSIM18 was grown to mid-log phase at 30 C in LB + 200 g/mL hygromycin, then expression of the lambda Red proteins was induced by incubating the cells at 42 C for 15 min, followed by 15 min on ice

et al., 2014

Chaturvedi P, Wiese AJ, Ghatak A, Zvesk Drbkov L, Weckwerth W, Honys D (2021) Heat stress response mechanisms in pollen development

The in vitro experiments have confirmed that vitamin D could inhibit the growth of Mtb and secretion of pro-inflammatory cytokines in peripheral blood monocytes (Martineau et al., 2007), stimulate the receptor-induced expression of antimicrobial peptides, and up-regulate LL37 gene to induce the autophagy-related proteins Atg5 and Beclin-1 to mediate the killing effect (Periyasamy et al., 2020)