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Antibodies Specific primary antibodies: Akt (2532 S, 1:2000), pS473-Akt (9271 S, 1:1000), S6K (9202 S, 1:2000), pT389-S6K (9205 S, 1:1000), Stat5 (9420 S, 1:10000), and pY694-Stat5 (9356 S, 1:1000) were obtained from Cell Signaling

Synaptic cleft width was determined from three synapses per MIA by six repeated measurements

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