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Rubin-Bejerano, I., Fraser, I., Grisafi, P

Kyoto, Japan) supplemented with 10% (v/v) fetal bovine serum (FBS), 10 mM 2-mercaptoethanol, 2 mM L-glutamine, 1 mM sodium pyruvate, 2 mM nonessential amino acids, 1% penicillin G/streptomycin, and 10 mM HEPES (pH 7.0) in a humidified incubator at 37C with 5% CO 2

In 2015, a phase II randomized double-blind, placebo-controlled clinical trial of CDDO-ME was restarted with 120 CKD patients of Grade 3 or 4, excluding patients bearing risk factors for fluid overload (e.g., high-level BNP and history of heart failure), and the results nicely showed a significant increase in eGFR without any signs of fluid overload (TSUBAKI study

The real key to reliable, repeatable research data lies in how you handle them from that point on