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Finally, the EcoRV fragment of pL1136 plasmid (which contains the pbggcs expression cassette) was cloned into the pL0009 vector to obtain the pbggcs complementation plasmid ( pbggcs - comp )

The libraries were quantified using the KAPA Library Quantification Kit for Illumina sequencing platforms, following the qPCR Quantification Protocol Guide (KAPA BIOSYSTEMS, KK4854), and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies, 5067-5582)

Kojima Y, Downing K, Kundu R, Miller C, Dewey F, Lancero H, Raaz U, Perisic L, Hedin U, Schadt E, Maegdefessel L, Quertermous T, Leeper NJ

Cells in the body make glutathione to protect themselves from oxidative stress