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Ethics declarations Ethics approval and consent to participate The present study was approved by the Institutional Animal Ethics Committee, Capital Medical University, and complied with ethical regulations for animal research and testing

The element that follows a half-filled subshell must now put an electron in a previously occupied orbital, creating stronger, intraorbital electron repulsion

The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)