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Another approach is to use double or multiple strains to construct in vitro biofilms for research

Protein Kinases Modulation Protein kinases such as ERK, AMPK, GSK3, p38 MAPK, and PI3K/AKT can mediate Nrf2 phosphorylation which enhance Nrf2 stability, thereby promoting nuclear Nrf2 translocation and transactivation activity (Wei et al., 2013

When the cultures reached an OD 600 of 0.40.5, we added 5 mM GGE to them

During this process, acetyl-CoA produced by the pyruvate dehydrogenase (PDC) complex in muscle exceeds the processing capacity of the tricarboxylic acid (TCA) cycle