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Persistent vomiting, inability to tolerate oral intake, or signs of bowel obstruction require immediate evaluation

Finally, 50-l of stop solution was loaded into each well and the absorbance was measured at 450 nm using the Biotek Synergy Neo2 HTS Multi-Mode Microplate Reader

Add the diluent slowly down the inside wall of the vial rather than directly onto the lyophilized powder, and let it dissolve without vigorous shaking