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The purpose of this study was to identify and isolate novel Bacillus species from the gut of hybrid groupers ( Epinephelus fuscoguttatus Epinephelus lanceolatus ) that may be used as probiotics, as reports indicate that commercially available probiotics are ineffective because the majority come from non-fish sources (Lieke et al., 2020)

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Equivalent amounts of protein (20 g) from each sample were subjected to electrophoresis on a SDS-polyacrylamide gel and were then transferred onto polyvinylidene difluoride membranes for subsequent blotting with specific antibodies