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Sequencing analysis confirmed that the Sec and Cys codon(s) of hGPx3 gene was(were) changed to Cys and Ser codon(s), respectively and no undesired mutation was introduced during the cloning process

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The limitation of that study was the small number of patients, but the authors concluded that compared with healthy neonates, the HIE group had increased melatonin, SOD, and NO concentrations, and the combination of melatonin to therapeutic hypothermia in infants with HIE can improve neurodevelopmental outcome at 6 months of age with effective effects in reducing oxidative stress in terms of NO and SOD serum level reduction

Insufficient intake of vitamin D can be linked to common age-related problems