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After incubation, uncap the column and let the flow-through fraction flow out the bottom

Results Establishment of CRC Drug-Tolerant Persister Cell Models While modeling the response to the chemotherapeutic drug 5-FU in the CRC cell line HCT116, which has exquisite sensitivity, we observed that the vast majority of the cells were killed within 9 days of exposure to the drug at a concentration 10-fold higher than the IC 50 value ( Figure 1A )

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Other ingredients:microcrystalline cellulose, gelatin, stearic acid, magnesium steara te, and silica