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Methods Plasmids, enzymes and inhibitors Plasmids For expression in bacterial cells, synthetic codon-optimized genes in pGEX6P.1 (BioCat GmbH, Heidelberg, DE) were used

AFBO-GSH is the most critical metabolite in AFB 1 detoxification pathway in the liver of chicken [51]

Although significant differences in protein levels were not found between the UFMs in the current analyses, the observation from the larger validation cohort measured by Zhi et al (1139 type 1 diabetic individuals and 848 control individuals) may have benefited from the improved statistical power

Neither peptide has been linked to a higher risk of low blood sugar in people without diabetes