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In addition to the above-mentioned affinity tags, new affinity purification strategies are now described in literature for protein isolation and detection (see the corresponding references in Table 2 ) such as the Fh8 tag [see The Novel Fh8 Fusion System (Hitag)], cellulose-binding domains I, II, and III ( CBD ), the HaloTag , the dockerin domain Dock tag, and the avidin-like protein, Tamavidin tag

Subsequently, 20 L of GSH (final concentration of 5.0 mM) was added to the wells