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Ohishi T, Fukutomi R, Shoji Y, Goto S, Isemura M

These include altered choline metabolism, changes in neurotransmitter metabolism, defective DNA methylation with consequent poly (ADP-ribose) polymerase (PARP) overactivation and PARP-controlled cell death, excitotoxicity, up-regulation of re-entry to the cell division cycle, hypomethylation of myelin lipids and myelin basic protein, white matter changes, and the formation of homocysteine thiolactone and subsequent protein homocysteinylation (89)

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MHR in the same cells was evaluated by a spectrophotometric assay described by Board and Pidcock (1981), expressed as mol/g Hb/min, and ACP1 activity was measured by the method of Magenis, et al