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Even when cells are isolated according to their surface markers, single-cell transcriptomics has revealed significant heterogeneity between cells that share the same surface markers.68 69 Even though bioinformatic methods exist to adjust for these differences, these methods likely are not able to account for all the methylation differences attributable to cell proportion differences seen between groups.7072 Most EWAS report modest changes in methylation (eg, 20% change), after correcting for cell-subtype variation

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