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Ubiquitination assay GSCs were treated with 10 M MG132 or vehicle control for 6 h before collection

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2 Methods 2.1 Model Development and Description To model the effect of alcohol(captured here by Ethanol, EToH) on APAP-induced liver injury, the model MALD (see Remien, Sussman, and Adler 2014) is connected to a physical pool of CYP2E1 as in (Chien, Thummel, and Slattery 1997)