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This assay converts protein concentration measurements into Ct values via qPCR, subsequently reporting concentrations as normalized protein expression (NPX) on a log2 scale through a normalization procedure [30]

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However, LA increased hepatic cholesterol and reduced LDL receptor protein conversion regulatory factors compared with the model group despite reduced mRNA expression of hepatic cholesterol synthesis enzyme (HMG-CoAr) and blood levels of cholesterol and LDL