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This approach was chosen to streamline the extensive data processing required for hundreds of sample injections, as the well-defined, predictable in vitro cell culture matrix, combined with precise retention time matching, provided sufficient confidence for analyte identification

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Western blot (WB) analysis For WBs, cells were extracted in RIPA buffer (Sigma-Aldrich, USA), separated by 10% SDS-PAGE, and transferred onto a polyvinylidene difluoride membrane (Millipore, USA)