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The resulting clean reads were assembled into unigenes using Trinity software (v2.13.2) after Q20, Q30, GC-content and sequence duplication level of the clean reads were calculated

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The results from the 500-yr climate model simulation support the unusual nature of these recent trends: only 2.9% of the 13-yr TX10d trends from the climate model are larger than the observed 20022014 trend with the long-term linear trend removed (dashed blue line of Fig