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Besides, 4-Hydroxynonenal (4-HNE), a major product of lipid peroxidation, is reported to induce the generation of ROS, which will aggravate the lipid peroxidation and ferroptosis, so 4-HNE can be a marker of ferroptosis as well [22,23,24, 26]

Membranes were washed and then incubated at RT for 2 h with horseradish peroxidase-labelled secondary (1:5,000, anti-rabbit #7074, or anti-mouse #7076 Cell Signaling) diluted in a solution of 5% milk in TBST

10.1016/0163-7258(90)90094-i 3 Allende-VegaN.Marco BruallaJ.FalvoP.AlexiaC.ConstantinidesM.de MaudaveA

6a), suggesting that no noticeable side-effects were induced under these treatment conditions