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On the neurological side, the pro-inflammatory factor released by AT may have first caused ferroptosis in the vagus nerve by inhibiting of the NRF2-GPX4 pathway, resulting in disorders of the autonomic nervous system

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The tissues collected from each group were collected in 0.01 M PBS with protease inhibitors [2 g/mL aprotinin, leupeptin, pepstatin A, and 120 g/mL phenylmethanesulfonyl fluoride (PMSF)] and homogenized (Polytron RT MR3100) at 3 10-s intervals

In this comparison (which, however, did not compare tumor tissue with matching surrounding tissue, but, rather, compared two different sets of tissues), no significant difference in CBS mRNA levels was reported [55]