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MS2 spectra were acquired for the top 20 most abundant ions per cycle at a resolution of 45,000, an AGC target of 30%, an isolation window of 0.7 m / z and a normalized collision energy of 34

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The proposed methodology integrates mitochondrial health assessments utilising mitochondrial homeostasis biomarkers in tear fluid as a non-invasive diagnostic tool, tailored nutraceuticals and lifestyle adjustments