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cultured insulin-sensitive human HepG2 cells under high-glucose conditions and administered cocoa-derived polyphenols and epicatechin, which blocked tyrosine phosphorylation and lowered total IR, IRS-1, and IRS-2 levels through PI3K/AKT and AMPK pathway inhibition, thereby mitigating high-glucose-induced insulin signaling impairment that alters gluconeogenesis (Mary et al., 2002)

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The FAD cofactor acts as an electron carrier, facilitating the formation of the unsaturated double bond at the -carbon of the acyl chain

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