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Samples were briefly centrifuged (10 s) to collect the solution at the bottom of the tube, followed by the addition of 30 L of MTBSTFA containing 1% TBDMCS (Thermo Fisher)

All experiments were carried out in accordance with the European Community Council Directive of 24th November 1986 (86/609/ECC)

On the other hand, persistent hyperkalemia suppresses impulse generation leading to bradycardia and conduction blocks and eventually cardiac arrest 75

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