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Lipids were extracted by resuspending the cell pellet in 2 ml ice cold methanol, followed by addition of 700 L ice cold glass distilled water

As expected, the injection of antisense oligonucleotide to Ldlr -null mice decreases the hepatic Fmo3 gene expression, resulting in decreased mouse plasma TMAO thereby decreasing aortic lesion in western diet fed mice (Shih et al., 2015)