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J Clin Biochem Nutr 62(1):2038 Van Bel F, Shadid M, Moison RM, Dorrepaal CA, Fontijn J, Monteiro L, Van De Bor M, Berger HM (1998) Effect of allopurinol on postasphyxial free radical formation, cerebral hemodynamics, and electrical brain activity

Therefore, a decrease in Cu transport in the brain could lead to an increase in oxidative stress as a consequence of increased release of ions that contribute to the formation of free radicals, a decrease in endogenous antioxidant systems (CU/Zn-SOD), and an increase in iron accumulation by decreasing the ferroxidase activity of ceruloplasmin [344]

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Briefly, the thermocycling protocol followed a pre-incubation at 95C for 10 minutes, followed by 45 cycles of 3-Step amplification: 1) denature at 95C for 20 seconds