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The resulting tree topology reveals a distinct distribution of fitness states: while the backbone of the lineage is dominated by high-affinity clones, B-cells with lower affinities compared to the population mean are predominantly observed only at the final time steps (the leaves of the tree)

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Exposure of RAW 264.7 cells to LPS for four hours dramatically increased the secretion of IL-6 to 613.2 35.1 pg/ml and TNF- to 1556.3 23.3 pg/ml