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Purification of the crude by semi-preparative HPLC (eluent A: 0.1% TFA in water, eluent B: 0.1% TFA in CH 3 CN/water 4/1 v/v, 6 mL min 1 , 035% eluent B in 25 min, C18 column, detection at 215 nm, 30 C) furnished SetCys-ALKEPVHGA-MPA peptide 8a as a white solid after lyophilization (25.3 mg, 20%)

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CJC1295 has an identical effect as GHRH actions and can alter hypothalamic feedback by increasing IGFI