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13 , 655672, doi:10.1038/nrd4363 (2014)

Step 1 prepares the hairs for reshaping, while Step 2 secures the new structure

CD44 deletion was achieved by precomplexing two Cd44 single guide RNA (sgRNA) guides (300 pmoles of Cd44 sgRNA1 plus 300 pmoles of Cd44 sgRNA2) with 36.3 pmoles of Cas9 protein (IDT, 1081059)

In all PEG-fused animals, labeling was found ipsilateral to the repair within the rostrocaudal and mediolateral boundaries of typical labeling as defined by Unoperated Controls