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The following agents were used as positive controls: cytomix (known inflammatory-inducing agent, prepared using 500 ng/mL TNF-, 0.2 mg/mL lipopolysaccharide, 1% FCS, tissue exposure via liquid basal), Triton X-100 (cytotoxic at 10%, via liquid basal exposure) or IL-13 (a goblet cell hyper-, metaplasia agent at 10 ng/mL, via liquid basal exposure)

Haikerwal et al., 2015)

Furthermore, by reducing CCO activity and compromising oxidative phosphorylation, copper deficiency compels cardiomyocytes to rely more heavily on anaerobic glycolysis, thereby reinforcing the metabolic shift that underpins pathological reprogramming [104]