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Description

Dhillon N, Kopetz S , Pei BL, Fabbro ED, Zhang T, Bruera E

M2-FLAG pull-down assay To test the binding between arr1 and the SH3 domain of Src, arr1 (20 M) was incubated with 3-fold molar excess of V2Rpp for 30 minutes at room temperature, then 10 M of SH3-FLAG was added