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Rating: 4.0495 (28 reviews)

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Among the mutants, the S586A variant displayed increased catalytic efficiency and substrate affinity, which is attributed to decreased steric hindrance and increased hydrophobicity, as verified by the saturation mutagenesis at S586

RT-PCR and quantitative real-time PCR (qPCR) RT-PCR was employed to detect RNA-level deletion of Fam134b exon 4, generating amplified products of either 361 bp or 488 bp