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Differential abundance analysis was done with DESeq2 using non-rarefied reads and tree_glom (or tax_glom for cecum rRNA) function

Nat Rev Urol (2016) 13(5):26674

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Effective purification remains equally critical, as unreacted precursors and luminescent impurities can obscure or distort the intrinsic optical properties of CQDs, especially those synthesised from complex biological materials [79]