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Nonspecific bound proteins were washed out with buffer A supplemented with 25 mM imidazole (Thermo Scientific, A10221) and laccases were eluted with buffer A containing 250 mM imidazole

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30,42 Experimental Evidence from Medicinal Plants in Diabetic Wound Healing In vivo studies using streptozotocin (STZ)-induced diabetic rat and mouse models have demonstrated improved wound contraction, epithelialisation, and collagen deposition following treatment with medicinal plant extracts