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For each treatment condition, the SYTOX-to-mCherry-object ratio (WT, FSP1 GFP-P2A-BFP or lung cancer lines) or SYTOX-to-phase-object ratio (FSP1GFP expression lines) was plotted against the 24-h imaging interval and the average area under the curve was plotted as a function of drug concentration (for example, RSL3) using GraphPad Prism version 10