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The system allowed researchers to precisely map the ~10 mV voltage-dependence shifts and calculate exact activation time constants

DNA expression plasmids were extracted and were purified from bacterial cells with NucleoBond Extra Midi Plus kit (Macherey-Nagel, Germany) and they were stored at -20C until their use for transient transfection into mammalian cells

Ability for Stress and Hormone Regulation DSIP appears to influence the bodys stress response and endocrine function: Cortisol modulation: It may help balance stress hormones, reducing the impact of chronic stress or overtraining