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The potential role of nicotine in breast cancer initiation, development, angiogenesis, invasion, metastasis, and resistance to therapy

Chiao, Changzhi Li, Jenshan Lin, Robert H

- Nausea or stomach upset (common with oral supplements) - Mild skin rashes or itching (possible with topical use) - Temporary bloating or cramping These are usually mild and subside when dosage is adjusted or discontinued

The crystalized MTT products in the cultures were dissolved by the addition of dimethyl sulfoxide (Merck Millipore, Burlington, MA, USA) at 37 C 35 , and then the absorbance at 595 nm was measured using an ELISA microplate reader (Tecan, Zurich, Swiss)