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[DOI] [PMC free article] [PubMed] [Google Scholar] 649.Kim R, Emi M, Tanabe K

Finally, the level M construct pAGB1332 (contains xopA , hpaH , hrpX , hrpG* and hpaA-2xLinker-sfgfp ) was assembled with the end linker pICH79264 and the level M construct pAGB1327 ( hrp gene cluster with a deletion in hpaA ) or pAGB1362 ( hrp gene cluster with a deletion in hpaA and hpaB ) in the level P vector pICH75322 using Bsa I and ligase, thus resulting in the level P constructs pAGB1334 (contains hpaA-2xLinker-sfgfp and a deletion in hpaA ) and pAGB1374 (contains hpaA-2xLinker-sfgfp and a deletion in hpaA and hpaB )