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A possible mechanotransducer candidate that opposes excessive TRPV4 activation might be the K+-permeable TREK-1 channel

Briefly, the media were removed from the 96-well plate and replaced with 100 l of fresh culture RPMI 1640 medium without phenol red

Unfolding studies suggest that FgGST1 is a highly cooperative molecule because, during GdnHCl-induced denaturation, a simultaneous unfolding of the protein without stabilization of any partially folded intermediate is observed

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