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heat-stable -amylase and Na 2 SO 3 were used to analyze NDF ( Rumen sampling and analysis On the last day of the study, approximately four hours after the morning feeding, ruminal fluid (9.8 mL) was collected using a stomach tube ( 2 SO 4 ) in the mobile phase of HPLC

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Persistent or severe side effects warrant immediate cessation and re-evaluation of the protocol and peptide source

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Then, lysates were incubated with anti-STAT1 or anti-flag antibody (normal IgG was used as a control) at 4 C overnight

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