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Cells were incubated for 10 min at room temperature and then 20 L of 10% CHAPS detergent (Sigma-Aldrich, C3023) was added, and cells incubated at room temperature for an additional 10 min to lyse cells

In addition, butylmalonate, an inhibitor of DIC, significantly decreased mGSH, suggesting DIC as the major GSH transporter in mouse cerebral cortical mitochondria (Kamga et al., 2010)