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Acknowledgements This study was funded by LogicBio Therapeutics (now part of Alexion, AstraZeneca Rare Disease)

Epidemiologic studies have consistently shown a mortality advantage among patients with hematocrits in the 30% to 36% range, and the NKF-DOQI Work Group on Anemia Management recommended a target hematocrit of 33% to 36% based on the expert panels' detailed literature review

The latter compound then undergoes oxidative decarboxylation catalyzed by the YUC family of flavin monooxygenases to produce IAA

Polymerization occurred at 37 C for 30 min, culture medium was overlaid and image capture performed as previously described (protocols.io For Boyden chamber invasion assays, cells were incubated in 0.1% inactivated BSA for 24 h and then plated in Matrigel-covered Boyden chambers (6 g/96-well, bottom treated with collagen type I) and incubated for 8 h at 37 C and 5% CO 2 atmosphere, with 5 ng/mL of epithelial growth factor as a chemoattractant