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For the experiments requiring medium adaptation, RPMI 1640 medium without L-glutamine (Life Technologies, Cat#: 21870076) was used and supplemented with L-glutamine (Thermo Fisher Scientific, Waltham, MA, Cat#: A2916801) to achieve the desired concentrations

Examination of novel zinc-binding groups for use in matrix metalloproteinase inhibitors

Based on the current studies, these relationships can be categorized into three confidence levels to clearly distinguish between validated, indirectly supported, and speculative associations (Figure 3)

And that matters, especially for mature skin: Regional conditions can influence how potent or soothing an ingredient is, which becomes more important as skin gets drier, thinner, and more reactive with age