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The global market for GLP-1 receptor agonists is expected to grow at a rate of 1518% per year, and reach more than $20 billion by 2028

Researchers at the University of Zagreb, Croatia, where BPC-157 was first isolated and characterized in 1993, have conducted the majority of preclinical studies

doi:10.1146/annurev.pharmtox.45.120403.095857 [PubMed] 15

Briefly, cells were seeded onto 24-well chamber slides, rinsed with ice-cold PBS and methanol at 20 C for 10 min, and then permeabilized with 0.1% Triton X-100