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Then 1 g of trypsin was brought up in 20 l of 100 mM TEAB and added to the S-Trap, followed by an additional 20 l of TEAB to ensure the sample did not dry out

Cholesterol can not only induce ectopic fatty acids accumulation, reshape an immunosuppressive microenvironment, activate hepatic stellate cells, and influence membrane fluidity or protein function, to further promote tumorigenesis in HCC but also activate NK cell proliferation or recruitment, and promote CD44 translocation into lipid rafts, so that prohibit HCC (Zhou and Sun, 2021)

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In addition, understanding the effect of maternal nutrient supplementary intake on breast milk composition seems important, because almost all pregnant and lactating mothers receive supplements